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Evident Corporation multi photon microscope
Multi Photon Microscope, supplied by Evident Corporation, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/multi+photon+laser+scanning+microscope/FVMPE-RS+Multiphoton+Laser+Scanning+Microscope/pm40214608-61-7-9
Average 97 stars, based on 1 article reviews
multi photon microscope - by Bioz Stars, 2026-09
97/100 stars

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Related Articles

Control:

Article Title: Multicompartmentalized Microvascularized Tumor-on-a-Chip to Study Tumor-Stroma Interactions and Drug Resistance in Ovarian Cancer.
Article Snippet: .. A similar approach was taken to study the differences in collagen fiber orientation in the different stromal scenarios for both control and halofuginone-treated groups in the microfluidic device by second harmonic generation microscopy (SHG) using FLUOVIEW FVMPE-RS Multi Photon Laser Scanning Microscope (Olympus). .. A tunable laser (InSight, SpectraPhysics) was used at 840 nm and z-stacks of 50 μm with a step size of 1 μm in 2 different positions of the stromal chamber in the microfluidic device were acquired using 25x water-immersion objective.

Microscopy:

Article Title: Multicompartmentalized Microvascularized Tumor-on-a-Chip to Study Tumor-Stroma Interactions and Drug Resistance in Ovarian Cancer.
Article Snippet: .. A similar approach was taken to study the differences in collagen fiber orientation in the different stromal scenarios for both control and halofuginone-treated groups in the microfluidic device by second harmonic generation microscopy (SHG) using FLUOVIEW FVMPE-RS Multi Photon Laser Scanning Microscope (Olympus). .. A tunable laser (InSight, SpectraPhysics) was used at 840 nm and z-stacks of 50 μm with a step size of 1 μm in 2 different positions of the stromal chamber in the microfluidic device were acquired using 25x water-immersion objective.

Article Title: Single-Frame Vignetting Correction for Post-Stitched-Tile Imaging Using VISTAmap.
Article Snippet: .. Briefly, these images were acquired using a multi-photon microscope (Olympus FVMPE-RS) equipped with a 20× objective lens (NA 0.6). ..

Article Title: Fluorescence visualization of endoplasmic reticulum Carboxylesterase in living cells with a small-molecule probe
Article Snippet: The uorescence spectra were recorded on a F-7000 uorescence spectrophotometer (Hitachi). .. Living cell imagings were obtained from an Olympus FV1000-MPE multi-photon laser scanning confocal microscope (Japan). ..

Laser-Scanning Microscopy:

Article Title: Multicompartmentalized Microvascularized Tumor-on-a-Chip to Study Tumor-Stroma Interactions and Drug Resistance in Ovarian Cancer.
Article Snippet: .. A similar approach was taken to study the differences in collagen fiber orientation in the different stromal scenarios for both control and halofuginone-treated groups in the microfluidic device by second harmonic generation microscopy (SHG) using FLUOVIEW FVMPE-RS Multi Photon Laser Scanning Microscope (Olympus). .. A tunable laser (InSight, SpectraPhysics) was used at 840 nm and z-stacks of 50 μm with a step size of 1 μm in 2 different positions of the stromal chamber in the microfluidic device were acquired using 25x water-immersion objective.

Article Title: Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures
Article Snippet: Animals were then allowed to recover before habituation in the head-fixed system (Neurotar Ltd.). .. Imaging data were acquired on an FV1200MPE multi-photon laser scanning microscope with a 25× immersion objective (XLPLN25XWMP2, Olympus) and adapted to allow a head-fixed system (Neurotar Ltd.). iGABASnFR and iGluSnFR were excited at 920 nm with a Ti-sapphire Chameleon Ultra pulsed laser (Coherent), and emission fluorescence was collected via three photomultipliers (PMTs) and filters (PMT 1: 450–500 nm; PMT 2: 515–560 nm; PMT 3: 590–650 nm). iGluSnFR and iGABASnFR imaging was performed by using a spiral line scan at 40–60 Hz at 320 × 320 pixels (512 × 512 μm) resolution and laser power was kept constant within each experiment. ..

Article Title: Fish Oil Supplement Mitigates Muscle Injury In Vivo and In Vitro: A Preliminary Report.
Article Snippet: In brief, C2C12 cells were cultured on 35 mm glass-bottomed petri dishes (MatTek, Ashland, MA, USA) and then washed and transitioned into modified Tyrode’s solution (140 mM NaCl, 5 mM KCl, 2 mM MgCl2, and 10 mM HEPES (pH 7.2). .. Membrane injury was induced using a FluoView FV1000 multi-photon confocal laser-scanning microscope (Olympus, Center Valley, PA, USA) on cells in Tyrode’s solution supplemented with 2.5 μM FM4-64 dye (Invitrogen, Eugene, OR, USA). .. A circular region of interest was selected along the edge of the plasma membrane and irradiated at 50% of maximum infrared laser power for 5 s. Pre- and post-damage images were captured every 3 s for a total of 60 s. The extent of membrane damage was analyzed using Fiji ImageJ software, Version 20170530 (National Institute of Health, Bethesda, MD, USA), measuring the fluorescence intensity encompassing the site of damage with results represented as ∆F/F0, as previously described [41].

Article Title: Development of an Asymmetric Hydrophobic/Hydrophilic Ultrathin Graphene Oxide Membrane as Actuator and Conformable Patch for Heart Repair
Article Snippet: .. Intravital Imaging of Vasculature: On days 1 and 7 after transplantation, mice were injected with Rhodamine B isothiocyanate-dextran (Sigma) (1 g L–1) solution (red) through the tail vein and anesthetized with isoflurane subsequently, then the fluorescent signal of the vascular structure within dorsal window chambers was captured by the multi-photon laser scanning microscopy (Olympus, FV1200MPE, Japan). ..

Article Title: Nanodysferlins support membrane repair and binding to TRIM72/MG53 but do not localize to t-tubules or stabilize Ca 2+ signaling.
Article Snippet: Muscle bundles were mechanically separated at the tendon and then adhered on glass-bottomed culture dishes (MatTek, Ashland, MA, USA) with Liquid Bandage (New Skin, Tarrytown, NY, USA) applied to the exposed tendons. .. Membrane disruption was induced in Tyrode supplemented with 2.5 mM FM4-64 dye (Thermo Fisher Scientific, Waltham, MA, USA), and in either 2 mM Ca2+ or 2 mM EGTA, with a FluoView FV1000 multi-photon confocal laser-scanning microscope (Olympus, Center Valley, PA, USA). .. A 2.5-mm circular region of interest was selected along the edge of the sarcolemma and irradiated with a DeepSee MaiTai titanium-sapphire laser at a wavelength of 800 nm and 10% of maximum infrared laser power for 3 s. Images were captured before and after damage every 5 s for a total of 60 s. Images were analyzed with ImageJ Fiji (National Institutes of Health, Bethesda, MD, USA), by measuring the Fo intensity in a region of interest encompassing the entire area of dye uptake, with results represented asDF/Fo.

Article Title: Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures.
Article Snippet: Animals were then allowed to recover before habituation in the head-fixed system (Neurotar Ltd.). .. Simultaneous imaging and ECoG recordings Imaging data were acquired on an FV1200MPE multi-photon laser scanning microscope with a 25× immersion objective (XLPLN25XWMP2, Olympus) and adapted to allow a head-fixed system (Neurotar Ltd.). iGABASnFR and iGluSnFR were excited at 920 nm with a Ti-sapphire Chameleon Ultra pulsed laser (Coherent), and emission fluorescence was collected via three photomultipliers (PMTs) and filters (PMT 1: 450–500 nm; PMT 2: 515–560 nm; PMT 3: 590–650 nm). iGluSnFR and iGABASnFR imaging was performed by using a spiral line scan at 40–60 Hz at 320 × 320 pixels (512 × 512 μm) resolution and laser power was kept constant within each experiment. ..

Imaging:

Article Title: Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures
Article Snippet: Animals were then allowed to recover before habituation in the head-fixed system (Neurotar Ltd.). .. Imaging data were acquired on an FV1200MPE multi-photon laser scanning microscope with a 25× immersion objective (XLPLN25XWMP2, Olympus) and adapted to allow a head-fixed system (Neurotar Ltd.). iGABASnFR and iGluSnFR were excited at 920 nm with a Ti-sapphire Chameleon Ultra pulsed laser (Coherent), and emission fluorescence was collected via three photomultipliers (PMTs) and filters (PMT 1: 450–500 nm; PMT 2: 515–560 nm; PMT 3: 590–650 nm). iGluSnFR and iGABASnFR imaging was performed by using a spiral line scan at 40–60 Hz at 320 × 320 pixels (512 × 512 μm) resolution and laser power was kept constant within each experiment. ..

Article Title: Development of an Asymmetric Hydrophobic/Hydrophilic Ultrathin Graphene Oxide Membrane as Actuator and Conformable Patch for Heart Repair
Article Snippet: .. Intravital Imaging of Vasculature: On days 1 and 7 after transplantation, mice were injected with Rhodamine B isothiocyanate-dextran (Sigma) (1 g L–1) solution (red) through the tail vein and anesthetized with isoflurane subsequently, then the fluorescent signal of the vascular structure within dorsal window chambers was captured by the multi-photon laser scanning microscopy (Olympus, FV1200MPE, Japan). ..

Article Title: Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures.
Article Snippet: Animals were then allowed to recover before habituation in the head-fixed system (Neurotar Ltd.). .. Simultaneous imaging and ECoG recordings Imaging data were acquired on an FV1200MPE multi-photon laser scanning microscope with a 25× immersion objective (XLPLN25XWMP2, Olympus) and adapted to allow a head-fixed system (Neurotar Ltd.). iGABASnFR and iGluSnFR were excited at 920 nm with a Ti-sapphire Chameleon Ultra pulsed laser (Coherent), and emission fluorescence was collected via three photomultipliers (PMTs) and filters (PMT 1: 450–500 nm; PMT 2: 515–560 nm; PMT 3: 590–650 nm). iGluSnFR and iGABASnFR imaging was performed by using a spiral line scan at 40–60 Hz at 320 × 320 pixels (512 × 512 μm) resolution and laser power was kept constant within each experiment. ..

Fluorescence:

Article Title: Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures
Article Snippet: Animals were then allowed to recover before habituation in the head-fixed system (Neurotar Ltd.). .. Imaging data were acquired on an FV1200MPE multi-photon laser scanning microscope with a 25× immersion objective (XLPLN25XWMP2, Olympus) and adapted to allow a head-fixed system (Neurotar Ltd.). iGABASnFR and iGluSnFR were excited at 920 nm with a Ti-sapphire Chameleon Ultra pulsed laser (Coherent), and emission fluorescence was collected via three photomultipliers (PMTs) and filters (PMT 1: 450–500 nm; PMT 2: 515–560 nm; PMT 3: 590–650 nm). iGluSnFR and iGABASnFR imaging was performed by using a spiral line scan at 40–60 Hz at 320 × 320 pixels (512 × 512 μm) resolution and laser power was kept constant within each experiment. ..

Article Title: Extracellular glutamate and GABA transients at the transition from interictal spiking to seizures.
Article Snippet: Animals were then allowed to recover before habituation in the head-fixed system (Neurotar Ltd.). .. Simultaneous imaging and ECoG recordings Imaging data were acquired on an FV1200MPE multi-photon laser scanning microscope with a 25× immersion objective (XLPLN25XWMP2, Olympus) and adapted to allow a head-fixed system (Neurotar Ltd.). iGABASnFR and iGluSnFR were excited at 920 nm with a Ti-sapphire Chameleon Ultra pulsed laser (Coherent), and emission fluorescence was collected via three photomultipliers (PMTs) and filters (PMT 1: 450–500 nm; PMT 2: 515–560 nm; PMT 3: 590–650 nm). iGluSnFR and iGABASnFR imaging was performed by using a spiral line scan at 40–60 Hz at 320 × 320 pixels (512 × 512 μm) resolution and laser power was kept constant within each experiment. ..

Membrane:

Article Title: Fish Oil Supplement Mitigates Muscle Injury In Vivo and In Vitro: A Preliminary Report.
Article Snippet: In brief, C2C12 cells were cultured on 35 mm glass-bottomed petri dishes (MatTek, Ashland, MA, USA) and then washed and transitioned into modified Tyrode’s solution (140 mM NaCl, 5 mM KCl, 2 mM MgCl2, and 10 mM HEPES (pH 7.2). .. Membrane injury was induced using a FluoView FV1000 multi-photon confocal laser-scanning microscope (Olympus, Center Valley, PA, USA) on cells in Tyrode’s solution supplemented with 2.5 μM FM4-64 dye (Invitrogen, Eugene, OR, USA). .. A circular region of interest was selected along the edge of the plasma membrane and irradiated at 50% of maximum infrared laser power for 5 s. Pre- and post-damage images were captured every 3 s for a total of 60 s. The extent of membrane damage was analyzed using Fiji ImageJ software, Version 20170530 (National Institute of Health, Bethesda, MD, USA), measuring the fluorescence intensity encompassing the site of damage with results represented as ∆F/F0, as previously described [41].

Article Title: Nanodysferlins support membrane repair and binding to TRIM72/MG53 but do not localize to t-tubules or stabilize Ca 2+ signaling.
Article Snippet: Muscle bundles were mechanically separated at the tendon and then adhered on glass-bottomed culture dishes (MatTek, Ashland, MA, USA) with Liquid Bandage (New Skin, Tarrytown, NY, USA) applied to the exposed tendons. .. Membrane disruption was induced in Tyrode supplemented with 2.5 mM FM4-64 dye (Thermo Fisher Scientific, Waltham, MA, USA), and in either 2 mM Ca2+ or 2 mM EGTA, with a FluoView FV1000 multi-photon confocal laser-scanning microscope (Olympus, Center Valley, PA, USA). .. A 2.5-mm circular region of interest was selected along the edge of the sarcolemma and irradiated with a DeepSee MaiTai titanium-sapphire laser at a wavelength of 800 nm and 10% of maximum infrared laser power for 3 s. Images were captured before and after damage every 5 s for a total of 60 s. Images were analyzed with ImageJ Fiji (National Institutes of Health, Bethesda, MD, USA), by measuring the Fo intensity in a region of interest encompassing the entire area of dye uptake, with results represented asDF/Fo.

Transplantation Assay:

Article Title: Development of an Asymmetric Hydrophobic/Hydrophilic Ultrathin Graphene Oxide Membrane as Actuator and Conformable Patch for Heart Repair
Article Snippet: .. Intravital Imaging of Vasculature: On days 1 and 7 after transplantation, mice were injected with Rhodamine B isothiocyanate-dextran (Sigma) (1 g L–1) solution (red) through the tail vein and anesthetized with isoflurane subsequently, then the fluorescent signal of the vascular structure within dorsal window chambers was captured by the multi-photon laser scanning microscopy (Olympus, FV1200MPE, Japan). ..

Injection:

Article Title: Development of an Asymmetric Hydrophobic/Hydrophilic Ultrathin Graphene Oxide Membrane as Actuator and Conformable Patch for Heart Repair
Article Snippet: .. Intravital Imaging of Vasculature: On days 1 and 7 after transplantation, mice were injected with Rhodamine B isothiocyanate-dextran (Sigma) (1 g L–1) solution (red) through the tail vein and anesthetized with isoflurane subsequently, then the fluorescent signal of the vascular structure within dorsal window chambers was captured by the multi-photon laser scanning microscopy (Olympus, FV1200MPE, Japan). ..

Disruption:

Article Title: Nanodysferlins support membrane repair and binding to TRIM72/MG53 but do not localize to t-tubules or stabilize Ca 2+ signaling.
Article Snippet: Muscle bundles were mechanically separated at the tendon and then adhered on glass-bottomed culture dishes (MatTek, Ashland, MA, USA) with Liquid Bandage (New Skin, Tarrytown, NY, USA) applied to the exposed tendons. .. Membrane disruption was induced in Tyrode supplemented with 2.5 mM FM4-64 dye (Thermo Fisher Scientific, Waltham, MA, USA), and in either 2 mM Ca2+ or 2 mM EGTA, with a FluoView FV1000 multi-photon confocal laser-scanning microscope (Olympus, Center Valley, PA, USA). .. A 2.5-mm circular region of interest was selected along the edge of the sarcolemma and irradiated with a DeepSee MaiTai titanium-sapphire laser at a wavelength of 800 nm and 10% of maximum infrared laser power for 3 s. Images were captured before and after damage every 5 s for a total of 60 s. Images were analyzed with ImageJ Fiji (National Institutes of Health, Bethesda, MD, USA), by measuring the Fo intensity in a region of interest encompassing the entire area of dye uptake, with results represented asDF/Fo.



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